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Background: Dengue fever is one of the Indonesia’s health problem. This fever is caused by Aedes aegypti mosquito species as the main vector of dengue virus to spread. Preventing action can be done by using insecticides. Synthetic insecticide has bad impacts for the environment and human health. Lemon basil leaves (Ocimum citriodorum) has active components so it potential as biological insecticide. This research was conducted to study the larvacide activity of lemon basil leaves against Aedes aegypti larvae.
Methods: Aedes aegypti third larvae instar stage were used in this study. The larvacide activity was being carried out by different concentration of basil leaves (4%, 4,2%, 4,4%, 4,6%, 4,8%, 5%). The numbers of death larvae on each treatment were counted. Statistical test performed by ANNOVA and linear regression using SPSS version 15.0 program.
Results: Lemon basil leaf extract 5.0% resulted in the highest mortality of mosquito larvae (48.75%). ANNOVA statistical test showed that there was a significant effect of lemon basil leaf extract against Aedes aegypti mosquito larvae. Linear regression analysis showed that the larvicidal lemon basil leaf extract is 61.1%.
Conclusion: Lemon basil leave extracts have larvacide ability to kill Aedes aegypti larvae. Further studies may be conducted to know the LC 50 concentration of lemon basil leave extract which can be developed as a biological insecticide.